細(xi)胞融合儀(yi)配(pei)件(jian)-貼(tie)壁細胞轉基(ji)因(yin)電極(ji)

細(xi)胞融合儀(yi)配(pei)件(jian)-貼(tie)壁細胞轉基(ji)因(yin)電極(ji)PP35-2P電極(ji) 是(shi)設(she)計(ji)用(yong)於(yu)原位貼(tie)壁細胞的電穿(chuan)孔(kong)或(huo)在(zai)除樣(yang)品(pin)杯(bei)以(yi)外(wai)的其他容器內(nei)進(jin)行較大量(liang)的(de)電穿(chuan)孔(kong)操(cao)作(zuo)，使用(yong)電極(ji)在(zai)原位對(dui)貼(tie)壁細胞進行電穿(chuan)孔(kong)避(bi)免了(le)需(xu)使用(yong)化(hua)學(xue)品消(xiao)化(hua)細胞以(yi)及低(di)穿(chuan)孔(kong)效率(lv)，中斷了細(xi)胞周(zhou)期(qi)及細胞間(jian)的等諸多(duo)問題．貼(tie)壁細胞轉基(ji)因(yin)電極(ji)PP35-2P電極(ji) 應(ying)用(yong)於(yu)哺乳(ru)動(dong)物(wu)細胞轉染(ran)，基(ji)因(yin)治(zhi)療，蛋白或藥物(wu)的(de)傳(chuan)遞(di)，也(ye)可(ke)應(ying)用(yong)於(yu)植物(wu)和酵(jiao)母(mu)。

細胞融合儀(yi)配(pei)件(jian)-貼(tie)壁細胞轉基(ji)因(yin)電極(ji)是(shi)壹種可復用(yong)的電極(ji)，適(shi)用(yong)於(yu)配(pei)裝(zhuang)於(yu)6孔(kong)板(ban)的(de)每(mei)個(ge)單孔(kong)或(huo)壹(yi)個(ge)35mm直(zhi)徑(jing)的(de)培(pei)養皿(min)，由13個(ge)間(jian)隔2mm的(de)鍍(du)金(jin)電極(ji)組成，適用(yong)於(yu)BTX各型(xing)電穿(chuan)孔(kong)儀(yi)。

Technical 


Standard Capabilities
Voltage Range: 0 - 300V
Pulse Length Range: 1 - 35 msec in PBS
Volume Range: 0.5 - 3.0 ml
Physical Characteristics
Weight: 6 oz
Number of Electrodes: 13
Electrode Thickness: 0.5 mm
Electrode Material: Gold Plated.
Generator Compatibility ECM 830, 630, 600, 399, 395, T 820

Ordering 


PP35-2P Petri Pulser PP35-2P 

References

Marrero et al., Journal of Biological Chemistry, 270 (26):15734-15738 (1995)
Scheiffer et al., Journal of Biological Chemistry, 271 (17):10329-10333 (1996)
Marrero et al., Journal of Biological Chemistry, 272 (39):24684-24690 (1997)

BTX Protocols PR0165 and PR0330 (1998)
BTX Protocols PR0290, PR00286, PR0359, PR0360, PR0364 (1998)