腦(nao)立體定(ding)位儀的實(shi)驗(yan)內(nei)容(rong)說明(ming)
點擊次數(shu):1464 更新(xin)時(shi)間(jian):2022-07-21
腦(nao)立體定(ding)位儀又稱腦(nao)固定(ding)裝置(zhi)(英(ying)文名:stereotaxicapparatus),它是利用顱骨外(wai)面(mian)的標誌(zhi)或其它參(can)考(kao)點(dian)所規(gui)定(ding)的三(san)度(du)坐標系統(tong),來確(que)定(ding)皮(pi)層下(xia)某些神經(jing)結(jie)構的位置(zhi),以(yi)便(bian)在非(fei)直(zhi)視暴(bao)露下(xia)對(dui)其進(jin)行(xing)定(ding)向的刺激、破(po)壞(huai)、註(zhu)藥物(wu)、引導(dao)電(dian)位等研(yan)究。
腦(nao)立體定(ding)位儀的實(shi)驗(yan)內(nei)容(rong)說明(ming):
(1)動物(wu)麻(ma)醉(zui):小(xiao)鼠,體重20-30g,稱(cheng)重(zhong)後以0.4%腹腔麻醉(zui)註(zhu)射(she)時(shi)必(bi)須(xu)緩慢,隨(sui)時(shi)註(zhu)意動(dong)物狀(zhuang)態。
(2)小(xiao)鼠頭(tou)部(bu)固定(ding):將小(xiao)鼠的門(men)齒(chi)固定(ding)於(yu)腦(nao)定(ding)位儀上(shang)頜固定(ding)器(qi),然(ran)後把壹(yi)側(ce)的耳捧推(tui)入(ru)動(dong)物(wu)的外(wai)道後,使動物(wu)的頭(tou)在處(chu)於(yu)兩滑(hua)道正中。再(zai)將另壹(yi)耳捧推(tui)入(ru)另壹(yi)側(ce)的外(wai)耳道。這時(shi)觀察(cha)兩個耳棒(bang)的刻(ke)度壹(yi)致(zhi)後,將兩耳棒(bang)上(shang)的固定(ding)螺絲扭緊,在將牙齒(chi)固定(ding)器(qi)上(shang)壓鼻環壓下後扭緊(鼻環、耳棒(bang)的松(song)緊度調節適(shi)宜為(wei)好(hao)),這(zhe)時(shi)從各(ge)個方(fang)向推(tui)壓動物(wu)頭(tou)部(bu)"均(jun)不會出(chu)現移動(dong)。
(3)開顱(lu)鉆(zuan)孔(kong)前(qian)的備(bei)皮(pi):剪(jian)去動物頭(tou)部(bu)毛,用2%碘酒(jiu)及75%酒(jiu)精(jing)棉球作(zuo)頭(tou)部(bu)皮(pi)膚的消(xiao)毒,沿矢(shi)狀縫作(zuo)壹(yi)3cm長的皮(pi)膚切(qie)口,分(fen)離皮(pi)下組(zu)織(zhi),用雙氧水清(qing)潔顱骨表(biao)面(mian)的筋膜及肌肉(rou)並(bing)剝離(li),推(tui)開骨膜,暴(bao)露前(qian)囟、人字(zi)縫及矢狀(zhuang)縫。
(4)確(que)定(ding)標準中線:將金(jin)屬定(ding)位針(zhen)向下移(yi)動到(dao)矢(shi)狀(zhuang)縫(feng)上(shang)方(fang)後,再(zai)前(qian)後移動定(ding)位針(zhen),使定(ding)位針(zhen)定(ding)位到(dao)前(qian)囟。
(5)小(xiao)鼠海(hai)馬定(ding)位:用定(ding)位針(zhen)在前(qian)囟後2mm,矢狀縫(feng)旁(pang)開2.5mm處(chu)定(ding)位壹點,即(ji)為(wei)海(hai)馬的平(ping)面(mian)位置(zhi),然(ran)後在此(ci)點(dian)上(shang)用鉆(zuan)孔(kong)針(zhen)在顱(lu)骨上(shang)鉆(zuan)壹(yi)小(xiao)圓孔(kong)。
(6)註(zhu)射(she)藥(yao)物:小(xiao)鼠海(hai)馬則位於該圓(yuan)孔下2mm,將1ml註(zhu)射(she)器(qi)吸(xi)入(ru)藥(yao)物(wu)安(an)置(zhi)到(dao)MC-5微(wei)操(cao)作(zuo)儀(yi)上(shang),操作(zuo)儀(yi)器使註(zhu)射(she)器(qi)針(zhen)頭(tou)由小(xiao)鼠腦(nao)鉆(zuan)孔(kong)處下降2mm時(shi)完(wan)成藥物(wu)註(zhu)射(she)到(dao)小(xiao)鼠腦(nao)海(hai)馬處。
(7)制作(zuo)腦(nao)組(zu)織(zhi)切(qie)片:將小(xiao)鼠腦(nao)制作(zuo)成切(qie)片,顯(xian)微(wei)觀察(cha)小(xiao)鼠腦(nao)中(zhong)紅染料位置(zhi)來(lai)驗證小(xiao)鼠腦(nao)海(hai)馬中是(shi)否(fou)定(ding)位準確(que)。